Rapid assay system for cytotoxicity tests using 14C-leucine incorporation into tumor cells.
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منابع مشابه
Phytohemagglutinin-induced cytotoxic action of normal lymphocytes on cells in tissue culture using 14C-leucine incorporation.
Stimulation of normal peripheral blood lymphocytes (PBL) by phytohemagglutinin (PHA) and production of PHA-induced cytotoxicity were investigated. PBL transformation stimulated by PHA was measured by 3H-thymidine incorporation, and the cytotoxicity was assayed by measuring the reduction of 14C-leucine incorporation in to target cells after interaction with PHA-treated PBL. The cytotoxicity of P...
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Background: Many in vitro studies suggested that choline incorporation into many types of tumors is related to cell proliferation. Whether in vivo choline incorporation is related also to cell proliferation or not was my question. Therefore, the aim of this pilot study was to investigate the relationship of in vivo [methyl- 14C]-choline incorporation and in vivo [methyl-3H]- thymidine incorpora...
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1. Rat thyroid lobes were incubated for various periods of time in Krebs-Ringer bicarbonate containing [3H]leucine and either [1-14C]galactose or [1-14C]mannose. Radioactivity in soluble proteins was determined after their separation by sucrosegradient centrifugation. 2. The time-course of incorporation of label from [14C]mannose into soluble thyroid proteins was parallel to that observed for [...
متن کاملEstimation of cell damage in monolayer culture using 14C-leucine incorporation.
A comparison was made of 14C-leucine incorporation and cell viability of T24 human bladder carcinoma cells after treatment with Thio-Tepa, a potent alkylating agent. It was found that Thio-Tepa produced concentration-dependent damage in these cells and also that 14C-leucine incorporation correlated well with cell viability. 14C-leucine incorporation can be used as a reliable and rapid screening...
متن کاملEffect of ACTH on the incorporation of L-(U-14C) leucine into the brain and spinal cord in mice.
The studies of Selye 8 and numerous others',7,10 have established that increased release of adrenocorticotropic hormone (ACTH) from the pituitary gland, which causes increased secretion of cortical steroids, is the common pathway of the stress response. The effect of corticosteroids on the synthesis of proteins has been studied in many organs 11. The results of these investigations indicate the...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1980
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.131.261